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10.1186/s13071-014-0629-9 39

After the reduction, a second alkylation step was carried out by adding 5 l of 100 mM d5-NEM

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Chromatographic separation was conducted on a Brownlee SPP C18 column (2.1 mm 75 mm, 2.7 m particle size, Perkin Elmer, Waltham, MA) using mobile phase A (100% H2O containing 0.1% formic acid and 1% of 1 M NH 4 OAc) and B (1:1 acetonitrile:isopropanol containing 0.1% formic acid and 1% of 1 M NH 4 OAc)

[DOI] [PubMed] [Google Scholar] 20.Zhang Q., Luo C., Li Z., Huang W., Zheng S., Liu C., Shi X., Ma Y., Ni Q., Tan W., et al