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Scientific references National Center for Biotechnology Information

Rotate injection sites regularly to help prevent irritation and tissue buildup

Early in the morning, before you have any food and before you reach for your early bird pick-me-up cup of coffee seems to work for many people, but any time in the day (as long as you stay consistent!) should get the job done

(PubMed) Sanghvi TG, Harvey PW, Wainwright E

Withdraw the needle and apply light pressure with a cotton ball or clean tissue if there is any bleeding

Each stage of respiration, from glycolysis in the cytoplasm to the citric acid cycle and oxidative phosphorylation in mitochondria, depends on NAD+/NADH to carry reducing equivalents, thereby driving the production of cellular energy