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For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)

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Another possible explanation of the inhibitory effect of methylene blue of nicorandils analgesia may be due to serotonergic interaction rather than guanylyl cyclase inhibition

Tissue perfusion indices P-(v-a) CO 2 and SvO 2 were restored to and remained at normal values from 4 h after the start of resuscitation