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In fact, chronic ethanol exposure in rats led to reduced HIF-1 expression [34]

Using regular sterile water instead of bacteriostatic water provides no antimicrobial protection, allowing bacterial growth that contaminates the entire vial

Remove them by tapping and re-adjusting

Protein concentrations in lysates were determined by Protein Assay Dye Reagent (BioRad #5000006), diluted in 2x Laemmli sample buffer (BioRad Cat#1610737) with fresh -mercaptoethanol (BioRad #1610710), and heated at 95C for 5 minutes