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Previous studies showed that a decreased level of PPAR- is associated with oxidative stress development in a rat model of duodenal injury induced by MTX (Sayed et al

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Human Foreskin Fibroblast (HFF-1) Cell Culture The HFF-1 cell line (ATCC-SCRC-1041) was cultured in DMEM, supplemented with 12% (v/v) FBS, 100 IU/ml penicillin and 100 g/ml streptomycin