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10.1038/ncb2903 30 LinH.ZhangX.WangD.LiuJ.YuanL.LiuJ.et al (2021)

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Allow vials to reach room temperature for 15 to 30 minutes before opening to prevent condensation Disinfect rubber stoppers with alcohol swab and allow complete evaporation Draw the calculated volume of bacteriostatic water into syringe Angle needle against vial wall and slowly inject water down the side, allowing it to gently slide onto powder Never squirt water directly onto lyophilized powder as this damages peptide structure Gently swirl or roll vial between palms to dissolve

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Altered intestinal microbial flora and impaired epithelial barrier structure and function in CKD: the nature, mechanisms, consequences and potential treatment