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Pitfalls in the analysis of the physiological antioxidant glutathione (GSH) and its disulfide (GSSG) in biological samples: an elephant in the room

Cell viability assay Cell viability studies were conducted in 96-well format and assayed using AlamarBlue (Life Technologies) reagent as per the manufacturers instructions

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The similar values between the magnesium and insulin treatment groups may indicate possible similar glutathione potentiating mechanism in these treatment groups