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After 24 h, cells were treated with DMSO control or RSL3 (0.11.0 M) and incubated for 7 days to allow for tumor spheroid formation

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The answer depends on: Whether the insulin is human or animal-derived (animal-based insulin disqualifies you) Stability of your dosage Absence of recent dosage changes Those on oral medications (like Metformin) are generally eligible as long as blood glucose is controlled

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Respect all directions regarding medication, fasting, and observation for the safest outcome