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Equilibration buffer: 50mM Tris-HCl 8M Urea, pH 8.0 0.1 Sample loading : Clarified supernatant fraction 1400ml at room temperature Plain wash buffer : 50mM Tris-HCl, pH 8.0 0.1

Both use the same voltage curve (3.7V nominal, 4.2V full) but the charge profile can differ slightly

Generic liraglutide (Nevolat) is significantly cheaper, typically costing between 70 and 160 per month from UK online pharmacies