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Reconstitution & Dosing Protocol Educational guide for reconstitution and daily dosing Standard Titration Schedule (5 mL reconstitution 20 mg/mL) Frequency: Once daily or 5 days on / 2 days off

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Abstract Aims: 25-Hydroxyvitamin D [25(OH)VD] deficiency/inadequacy is a major public health issue affecting more than 1 billion people worldwide

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Preparation of Assay Solutions Preparation of 5% 5-Sulfosalicylic Acid (SSA) Solution Note: SSA is not included in this kit