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Chen et al., Size-dependent cytotoxicity study of ZnO nanoparticles in HepG2 cells

Similarly, childhood obesity has seen a steep increase, with rates for individuals aged 5-19 years climbing from 4% in 1975 to almost 20% by 2022

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Experiments were performed in the imaging medium (DMEM without pyruvate, fluorescent vitamins and phenol red with 5 mM glucose, 25 mM HEPES, pH 7.4 and 1% dialyzed FBS), or when cells were switched to the imaging medium without glucose but with 10 mM pyruvate