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The normal distribution of mitochondria was greater at 30 C and 38.5 C (87.5 6.43% and 82.68 6.43%, respectively) than at 41 C (39.02 8.87%

Measurement of Carnitine, TMAO, -butyrobetaine, d9-carnitine, d9-TMAO, and d9--butyrobetaine Sample preparation and quantification for carnitine and TMAO in healthy volunteers Fifty microliters of plasma and 50 L urine samples were extracted with 450 L and 950 L methanol containing 200 ng/mL isotopically labeled internal standards (d3-carnitine and d9-TMAO) respectively, and the extraction was performed using the Geno/Grinder 2010 (SPEX SamplePrep., Metuchen, NJ, USA) at 1000 rpm for 3 min

Interestingly, inhibitory effects of PPG on hypotaurine/taurine synthesis were found in wild-type hepatocytes

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Calibration curve The TRaQ-G sensor was assembled in vitro as described before using the purified HTmGold fusion protein