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For example, in the case of Enterococcus amplification culture, if the inoculation density is too low, the production strain may undergo genetic mutations and completely lose the ability to produce the target product [217]
Baker, T
Continue 64-80oz daily minimum
Additionally, proteins such as FER (Alvau et al., 2016), ZP3R/sp56 (Cheng et al., 1994), zonadhesin (Tardif et al., 2010), believed to be essential for fertilization based on in vitro experiments, do not exhibit significant phenotypes in vivo when knocked out (Okabe, 2018)
PMID: 23544831
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