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Spiking experiments into the plasma fraction prior to extraction showed that enzymes catalyzing the conversion of NADH to NAD+ are localized in plasma, while those affecting NAD + , NADP + and NADPH are associated with the cellular fraction (Supplementary Table 1, Sample Type 4)

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After washing sessions in PBS for 3 10 min, these were then incubated in donkey Alexa-488 and 594 conjugated secondary antibodies (1:100 dilutions, Thermofisher, UK) for 1 h at RT

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Growth hormone directly and indirectly stimulates protein synthesis while reducing protein breakdown in skeletal muscle tissue