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, Wragg, N.M., Wilson, S.L

For PDX tumors, frozen sections were established and stained with 10 M DCFH-DA and 20 M BODIPY 581/591 C11 for 30 min, and then washed twice by PBS

15 g of protein was loaded and separated by 8-10% SDS-PAGE, then transferred to PVDF membranes (Bio-Rad, USA) and blocked using 5% non-fat milk for 1 hour at room temperature