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To assess the functional activity of each variant, we performed SRE-luciferase assays on HEK293 cells overexpressing each variant, followed by DOP activation by its agonists deltorphin II (DII) or DPDPE during 5 hours

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The binding was performed at 4 C for 4 h incubated and subjected to IB analysis

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These factors can influence which operation is safest