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Rotate sites weekly to avoid irritation

1 Introduction Acinetobacter baumannii is an emerging pathogen causing opportunistic infections in healthcare facilities worldwide (Villegas and Hartstein, 2003)

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,2010 Jan;7(1 Pt 1):269-76

For the extraction of mRNA, tissue samples were homogenized in TRI Reagent (Molecular Research Center) using a TissueLyser II system (Qiagen) and then chloroform extracted, precipitated using isopropanol, washed with 75% ethanol, and reconstituted with DEPC-treated water