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Indeed, an increase in oxidative stress was identified only after the inhalation of nicotine-containing e-liquids, highlighting the role of nicotine in the development of ENDS-induced vascular dysfunction [24]

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Subcutaneous injection of high doses of nicotine in mice achieving plasma concentrations similar to those of heavy smokers also increased epidermal thickness although lower nicotine doses simulating the plasma levels of lighter smokers had the opposite effect 29

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