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Following rules helps one to avoid major problems

JP patent 59,151,894 Kono G, Harada M, Sugisaki K, Nishida M (1977) High glutathione-containing yeast

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To achieve antibody functionalization, we applied a sequential labelling strategy, including reduction with TCEP, and then the alkynes ( 19-22 ) were covalently attached to trastuzumab, allowing subsequent click chemistry for conjugation with TAMRA-PEG 3 -azide (Fig