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Thus, to further test the polarity of the later ciliating and mature ciliated expression states in the ALI cultured scRNA-seq dataset, we used the Velocyto pipeline 78 (applying default options) to identify unspliced and spliced mRNA reads for each gene in the original in vitro BAM files

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The base solution consisting of glycerol and propylene glycol was not found to be cytotoxic at any concentration.48 These results seem to suggest that the toxic effects of electronic cigarettes could be elicited by the added flavours

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