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The suspension was sonicated 1 h at 4 C (Branson 5510, 40 kHz), centrifuged (15 min, 14 000 g , 4 C) and 800 L supernatant transferred to a 1.5 mL tube
She took it all in, quick with intent, For soon they would be-as directed-elsewhere
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I mean he was known, but I didnt really know who he was, a certain somebody who had that hit Space Oddity in 69 which I barely registered with to be honest with you
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