elegans For sodium azide (NaN 3 ) stress assays, synchronised 1dayold adult (1DOA) hermaphrodites were exposed to 8 mM NaN 3 in M9 buffer (with 0.01% Triton X100) for 1 h on a shaking incubator at 20C
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Early conjectures proposed that it was caused by syphilis, without accurate evidence
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