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Cofactor and substrate binding induce the switch between the open and closed conformations of these domains
[DOI] [PMC free article] [PubMed] [Google Scholar] 83.Novak V., Milberg W., Hao Y., Munshi M., Novak P., Galica A., Manor B., Roberson P., Craft S., Abduljalil A
Tissue was homogenized as detailed above for respirometry studies
Methods Protein expression and purification The MmIGF1R with C terminal tail truncation and kinase dead mutation (residues 11262, Y951A, D1107N) was subcloned into the pEZT-BM expression vector
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