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82 Nicotine is responsible for an increased adhesion of planktonic bacteria to the tooth biofilm and its thickening due to an increase in the synthesis of extracellular polysaccharides (EPS), glucosyltransferase (Gtf) and glucan-binding protein (Gbp) at the mRNA and protein levels

They connect through narrow channels

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A three-stage (triple) cellulose acetate filter was used with a 15-mm mouth-end a 10-mm central section containing 20 mg of CR20 resin (distributed among the cellulose acetate fibres in a Dalmatian format), and a 12-mm section containing 50 mg of HAC in Dalmatian format at the tobacco-rod end of the cigarette