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In the case of histone H3, the peptide targeted the exposed N-terminal domain, known as a tail, which is known for undergoing post-translational modifications

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The resulting lysates were incubated with either magnetic beads conjugated to anti-Flag antibodies or protein A/G agarose beads coupled to the corresponding antibodies (Thermo Fisher Scientific, 88802) at 4 C for 3 h

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Orphanet J Rare Dis 15:59