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(5) Elute with approximately 5-10 bed volumes of Elution Buffer, collect fractions

Mertens J, Bregadze R, Mansur A, Askar E, Bickeboller H, Ramadori G et al

doi: 10.1016/j.celrep.2018.10.080 68 KochB.TravenA

The synthesis process carefully manages the incorporation of alpha-aminoisobutyric acid (Aib) at the N-terminus and the D-amino acids (D-2-naphthylalanine and D-phenylalanine), which do not occur in natural proteins but are critical to the peptides final properties

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Prados-Pardo , Martn-Gonzlez E, Mora S, Merchn A, Flores P, Moreno M