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When e-cigarettes first became available in 2006, they were heralded as a smokeless and therefore safer way to inhale nicotine, as well as a means to quit smoking

Gene expression levels of TNF, IL-6, IFN, and E-cadherin were measured using qRT-PCR

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Shih clarified that heated tobacco is classified as a designated tobacco product and requires a health risk assessment before approval for sale, unlike conventional cigarettes

Radioactivity was measured in single cartridges (4 per pack), and the content of two selected cartridges was mixed, which constituted one measurement sample, thus analyzing 8 cartridges from one pack