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Moreover, PI3K-Akt signaling was significantly enriched at mid- and late phases of iHLO development, implying the expansion and functional maturation of the liver organoid, along with structural organization and functional integration of different cell types within the organoid

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Administration ofthe ROS inhibitor N- acetylcysteine (NAC) partially restored GSH, attenuated ROS accumulation, and modestly alleviated Gln-deprivation-induced growth inhibition (Fig

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