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We have further observed that p-BSQ oxidized protein in the nitrogen atmosphere in the absence of molecular oxygen (4), indicating that there is a direct interaction of p-BSQ and biological macromolecules

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In case of smoke-induced cell death in human lung microvascular endothelial cells exposed to phosphate-buffered saline-conditioned cigarette smoke mimicking the exhaled cigarette smoke, the level of exposure was assessed by the increase in nicotine/cotinine levels, thus assessed cell injury and cell death was discussed in relation to the increase in nicotine/cotinine levels [14]