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Moreover, in order to clarify the previously proposed methodological problems of abstainers misclassification, sick quitter hypothesis, and potentially confounding factors 17 , we categorized drinking status of ex-drinkers into regular drinker to avoid misclassifying the former drinkers as abstainers and controlled 15 confounders to minimize confounding

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Samples were then analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) on a Hypercarb column (Thermo Scientific), using 10 mM ammonium acetate (with 0.01% formic acid) and methanol as mobile phase

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Generalization of conditioned fear-potentiated startle in humans: Experimental validation and clinical relevance