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The lysates (2 g of protein per lane) in RIPA buffer (Sigma-Aldrich, Cat# R0278) containing a protease inhibitor cocktail as well as phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich) were subjected to SDS-PAGE on 520% gradient gels (SuperSep Ace, FUJIFILM-Wako) followed by immunoblotting

Each cone holds about 1 gram of smokable herbs

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