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For carbonyl analysis, DNPH-coated glass fiber filters (ORBO 827, SUPELCO, CA, United States) followed by DNPH cartridges (Sep-Pak XPoSure Plus Short Cartridge, Waters, Milford, MA, United States) were used to assure collection of both particle- and gas-phase carbonyls ( Nicotine Analysis The GFF were extracted with 4 ml methanol
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Rigorous statistical criteria were employed to select metabolite candidates, and further use for pathway enrichment analyses
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