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-lipoic acid (LA), as an antioxidant, has been reported to play an important role in renal protection, but the underlying mechanism remains poorly explored

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Cells were cultured in MEM- medium supplemented with 5% FBS, 100 U/mL penicillin, 100 g/mL streptomycin, and 0.25 g/mL amphotericin B and were maintained in a humidified incubator at 37 C, 5% CO 2

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To this end, we first generated A375 cells stably expressing either mock (control) or FSP1-Flag and performed immunoprecipitation (IP) assays to isolate and purify the Flag-tagged protein (Fig