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There are two main forms of bilirubin in blood as determined by the Van den Bergh reactions (reaction with diazo dyes): Unconjugated (indirect): This is bound to albumin and is the dominant form of total bilirubin in blood

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However, as the results could not be reproduced by other groups [164], it was finally realized that the authors of the original study dissolved APAP in DMSO instead of saline

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