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Several studies have delineated the significant role of anxiety, depression, mental and psychological stress, and physical and sexual abuse as contributing factors in the irritable bowel syndrome (2, 5, 11)

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The NNAL calibration curve was generated based on concentrations of 10, 50, 100, 250, and 500 pg/mL and each standard solution was spiked with 200 pg/mL of 13 C 6 -NNAL

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