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The Real-Time PCR was performed with a CFX ConnectTM Real-Time PCR Detection System (BIO-RAD, Warsaw, Poland) and following cycling conditions: 95 C for 2 min, followed by 40 cycles at 95 C for 15 s, annealing for 15 s, and elongation at 72 C for 15 s

There are no significant restrictions, but you should avoid vigorously massaging the injection site for a few hours

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As of 1 January 2021, M2.2 (Intravenous infusions and/or injections of more than a total of 100 mL per 12-hour period) have now been classified as a Specified Method based on the newly introduced Article 4.2.2 in the 2021 Code, that makes it possible to identify a Prohibited Method as Specified