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Determination of conventional chemical compositions The contents of total sugar, reducing sugar, total nitrogen, total alkaloids, chlorine, potassium, protein were determined by a continuous flow analyzer (Alliance-Futura), according to Tobacco Industry Standards YC/T159-2002, YC/T 1612002, YC/T 4682013, YC/T 2172007, YC/T 1622011, YC/T2492008, respectively
Measurement of CO concentration was conducted continuously for 180 min, and every four minutes, the concentration observed in the detector screen was noted down
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