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10 L of the extracted protein samples were mixed with 3 L of a 5X sample buffer in microcentrifugation tubes, heated to 90 o C for 5 minutes, centrifuged (2,000 x g for 1 minute) and gel electrophoresis took place at 100 Volts for 60 minutes at room temperature
Side effects include increased heart rate and blood pressure, lung disease, chronic bronchitis and insulin resistance leading to type 2 diabetes
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