Primary monolayer cultures were trypsinized, re-suspended in agarose gel medium : DMEM/F-12 containing 10% FBS, 100 units/ml penicillin G, 100 mg/ml streptomycin, and 50 mg/ml ascorbate, to a concentration of 210 4 cells/ml, then mixed with equal volume of 1% low-temperature melting agarose (Sigma-Aldrich, Steinheim, Germany) in agarose gel medium, giving a final concentration of 110 4 cells/ml suspended in 0.5% low-temperature melting agarose in agarose gel medium (suspension agarose)
Appalachian raised and hailing from Boone, North Carolina, the five-piece band channels the rugged beauty and resilience of their mountain roots into every performance
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