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Unbound samples were removed with 60 mM imidazole, and the target protein was eluted with buffer containing 250 mM imidazole

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A further increase in the level of oxidative stress could ultimately trigger a cytotoxic response originating in the mitochondria and lead to cellular apoptosis or necrosis (Figure 2)

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[74], the results obtained were expected regardless of whether early exposure to APAP was responsible for most cases of ASD

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