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I believe that this is one of the most important findings in tobacco-related health in years, and it brings to light an issue, like e-cigarettes and secondhand smoke, that we in the health care field must address with urgency

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Because non-CpG sites can be methylated in mtDNA, which may reduce the PCR primer binding efficiency [45], we verified PCR product intensity on 1% agarose gel and inspected the peak heights in pyrosequencing readouts

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