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While nicotine itself clears the body relatively quickly, it metabolizes into a compound called cotinine

For the RAW264.7 experiments, cells in the logarithmic growth phase were seeded in well plate at a density of 1 10 5 cells/mL

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Fourth, cigarette smoke triggers the activation of pulmonary alveolar macrophages, leading to superoxide and hydrogen peroxide production and thereby contributing to the oxidative damage of DNA and RNA, increasing the likelihood of carcinogenesis 33