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Rats had already received intraperitoneal injections of nicotine at 1 mg/kg and MLA at 1 mg/kg once daily for 1 week before surgery and continued receiving intraperitoneal injections of 1 mg/kg nicotine or MLA in sterile saline once daily for 3 week (Teng et al., 2019), whereas matched control rats received an equal volume of intraperitoneal saline