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The incubation system contained 1 mg BSA and 50 l of smoke solution obtained from cigarettes with or without charcoal filter in a final volume of 200 l of 50 mM potassium phosphate buffer, pH 7.4

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Thromboxane is an important mediator of smoking-induced inflammation and has been implicated in the pathogenesis of cardiovascular disease 25

[4] If the fluid in the vape contains nicotine, then there is an increased risk of teeth stains and discoloration, teeth grinding, and receding gums