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Samples were permeabilized with 0.4 M glycine and 0.3% Triton X-100, and nonspecific binding was blocked with 2% normal swine serum (DAKO, Glostrup, Denmark)

RELATED: The story behind Toby Keith's hit 'Courtesy of the Red, White and Blue' File: Toby Keith performs on stage during the 2023 People's Choice Country Awards held at the Grand Ole Opry House on September 28, 2023 in Nashville, Tennessee

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