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Sperm preparation with microfluidic sperm sorting chip may improve intracytoplasmic sperm injection outcomes compared to density gradient centrifugation

The total number of the cells was evaluated based on the following formula: where Q was the total number of sexual lineage cells observed in all disectors, h was the optical disector's height, a/f was the area of the counting frame, P was the total number of observed frames, BA was the setting of the microtome, and t was the mean of the final section thickness [36]

The additive effect appears strongest in individuals with insulin resistance, where metformin's mechanism directly addresses a core metabolic defect