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Studies link e-cigarette restrictions to higher mortality rates, supporting their role in harm reduction

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after adding 500 L of -20 C pre-cooled 70% methanol aqueous internal standard extraction solution, samples were vortexed for 3 min (with steel beads added for an additional 3 min vortexing if incomplete dispersion was observed), sonicated in an ice-water bath for 10 min, vortexed again for 1 min, incubated at -20 C for 30 min, centrifuged at 4 C and 12,000 r/min for 10 min, and finally the entire supernatant was aspirated and filtered through a 0.22 m PTFE membrane for instrumental analysis under the following

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