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Research indicate that inactivity independently increases the risk of skeletal muscle wasting, osteoporosis [62], type 2 diabetes, cardiovascular disease and depression [63]

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Although our study did not employ quantified cotinine levels, as used by Polanska et al

The organic layer is analyzed by GC with a flame ionization detector

The trachea was exposed and cannulated, and 200 L of 4% formaldehyde was instilled into the left lung for in situ fixation