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After incubation with a horseradish peroxidase (HRP)-conjugated anti-mouse (W4021) or anti-rabbit (W4011) antibody (Promega, Fitchburg, WI), the immunoreactive bands were visualised using an enhanced chemiluminescence kit (BD Biosciences, Franklin Lakes, NJ) and imaged with an ImageQuant LAS-4000 system (GE Healthcare, Chicago, IL)