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The proteins were extracted and transferred to a nitrocellulose membrane through via three different methods: Semi- dry transfer machine (Trans-Blot SD Semi-Dry Transfer Cell, Bio-Rad), Wet transfer machine (Wet/Tank Blotting Systems, Bio-Rad), and Turbo-transfer machine (Trans-Blot Turbo Transfer Starter System, Bio-Rad)

Last weeks ruling included details on how to use these exceptions and what steps the shipper must take

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